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Applied scientific calculations
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Order
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Description
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1
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Explain the metric system - length/volume/mass.
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2
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Perform scientific calculations used to prepare solutions, buffers, and media: weight/volume; volume/volume/ molarity, percentage.
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3
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Perform scientific calculations used to prepare chemical and bacterial dilutions.
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4
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Explain the theory and use of micropipetting.
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Background survey of microbial media, solutions buffers, and applicable laboratory equipment
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Order
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Description
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1
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Explain the purpose of selected complex rich media and minimal media, selective and differential media.
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2
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Explain the purpose of biological buffers, pH scale, and the background of a pH meter.
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3
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Describe the various approaches used to store bacteria long-term or permanently.
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4
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Explain the theory and use of spectroscopy.
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5
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Explain the theory and use of centrifugation.
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Principles of organelle and protein isolation and enzyme assay
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Order
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Description
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1
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Explain the theory of differential centrifugation when isolating organelles (mitochondria).
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2
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Describe organelle structures and purpose.
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3
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Explain the purpose of the Bradford assay determining total protein content.
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4
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Explain the purpose of marker enzymes.
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5
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Explain assaying malate dehydrogenase (MDH) including substrates and products.
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6
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Explain calculating MDH rate and its specific activity.
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Background of protein columns and assay and protein gel analysis
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Order
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Description
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1
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Explain the rationale of size exclusion columns when purifying proteins.
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2
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Explain the theory and background of denaturing polyacrylamide gel electrophoresis (PAGE) when separating different proteins.
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3
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Explain the use of known molecular weight proteins in PAGE for the purpose of determining the molecular weight of an unknown protein.
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4
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Explain the use of known molecular weight proteins in PAGE for the purpose of determining the molecular weight of an unknown protein.
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Principles of plasmid DNA isolation and gel analysis
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Order
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Description
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1
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Explain the theory and use of agarose gel electrophoresis when analyzing deoxyribonucleic acids (DNA).
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2
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Describe the structure and purpose of prokaryotic plasmid DNA.
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3
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Explain the background of plasmid DNA isolation from bacteria.
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4
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Explain the results of agarose gels containing separated, differently sized plasmid DNAs.
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5
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Explain the use of a nucleic acid molecular weight standard for the purpose of determining the molecular weight of an unknown DNA fragment.
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6
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Describe how DNA is visualized within a gel and photo-documented.
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Theory of DNA restriction and analysis, ligation, transformation, and selection
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Order
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Description
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1
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Describe the purpose and mechanism of DNA restriction enzymes.
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2
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Explain the analysis of restricted DNA using agarose gel electrophoresis and a molecular weight standard.
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3
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Describe the purpose and use of DNA ligase.
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4
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Explain the theory and background of a chemical (calcium chloride) transformation using ligated plasmid DNA.
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5
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Explain the purpose of selection when transforming plasmid DNA.
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6
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Explain the use of proper transformation controls and scoring the final results of a transformation.
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